Folks
If you follow this plot at some point will you not start clipping the white point?
Yes, therefore intelligent use of the histogram is required in combination with the settings. With an Altair 183C capturing at 5000x3000 I usually push it and end up with a small number of pixels at or around 100%. Acceptable (to me) on a 20 mega-pixel frame. In line with Brian's suggestion, I have been easing up on the offset but maintain a centrally balanced bias frame to establish an offset value for a given gain.
For me it has been a year long journey to relate histogram to finished image. One thing I have become aware of is that by capturing what I believe is better data, the amount of processing required has significantly reduced - sometimes down to just a single 2-point 'S' shaped stretch in curves.
Using the Atik 314E CCD camera I recently acquired has been a revelation - only one setting,
exposure, so that really does focus in on the histogram.
I got a lot of value (and improvement) from reading Craig Stark (Nebulosity) articles on offset, bias and histograms. There is some really useful info in the Nebulosity manual (comes as part of the download).
Dave